The Transportation Gallery is a showcase for interesting and exciting vehicles from Discovery Park’s collection. This gallery is frequently updated as different vehicles, automotive accessories and parts are loaned for temporary display. One of the oldest cars in the gallery is a 1916 Ford Model T restored by the Ursery brothers of Union City, Tennessee, who restored many of the items seen on display at Discovery Park including the Titan I Missile in STEM Landing.

The most contemporary car on display is the 2007 Chevy Monte Carlo SS, otherwise known as the Budweiser #8 car. It was driven by Dale Earnhardt Jr. during his final season with Budweiser.  Along with the motorcycles, artifacts and memorabilia relating to the culture of transportation that can be seen, there are also many service station signs and vintage gas pumps from the Discovery Park collection, all restored to their original glory.